Is Template And Sense Strand The Same Thing?
Template vs Coding Strands
Information technology is often useful to distinguish the two strands of DNA -- the strand that is copied into mRNA and subsequently translated has the complementary sequence to the mRNA, while the base of operations sequence of the opposite strand directly corresponds to the codons in the mRNA.
The terms template strand, sense strand, and coding strand are ordinarily used to draw one of the two strands of DNA, yet the nomenclature is quite disruptive because different authors accept used these terms to describe both strands -- ane schoolhouse argues that the strand copied into mRNA should exist considered the template strand, merely the other school argues that the opposite strand which reflects the sequence in the mRNA should be considered the template because the corresponding codons are copied into protein. The commencement definition is used in the figures beneath, however, to avoid confusion, when using the words template, sense, or coding, information technology is essential to explicitly define how yous are using the terms. I believe that these terms are best defined as described below.
The term template strand refers to the sequence of Deoxyribonucleic acid that is copied during the synthesis of mRNA.
The opposite strand (that is, the strand with a base of operations sequence directly corresponding to the mRNA sequence) is chosen the coding strand or the mRNA-like strand considering the sequence corresponds to the codons that are translated into protein.
Although RNA polymerase must recognize sequences on the template strand, by convention we draw the Deoxyribonucleic acid sequence and regulatory signals on the "mRNA-like" strand. (This makes it simpler to directly determine the sequence of the resulting RNA.) The post-obit cartoon shows this concept for a hypothetical gene.
It may be useful to consider a existent gene as well. The Dna sequence of the phage P22 arc cistron and some of import regulatory sites is shown below. The upper strand of DNA is the "mRNA-like" strand. The lower strand is the strand that is complementary to the mRNA. The -35 region (TTGACA) and -x region (TATATT) of the promoter sequence and the transcriptional kickoff site (the A ) is indicated on the coding strand. Also note that the Deoxyribonucleic acid sequence of the coding strand corresponding to the RNA codons is shown in bold (of course, the T is a U in the RNA) -- the starting time codon is ATG the translational showtime site (fMet) and the final codon is TAA (Ochre) the translational stop codon.
References:
- Arc Deoxyribonucleic acid sequence from Genbank
- King, R., and W. Stansfield. 1985. A lexicon of genetics. Oxford University Printing, NY.
Please send comments, suggestions, or questions to smaloy@sciences.sdsu.edu
Concluding modified July 12, 2002
Is Template And Sense Strand The Same Thing?,
Source: http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/chroms-genes-prots/temp-strand.html
Posted by: hammerstherong1944.blogspot.com
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